![]() factor Protein A (ng/mL) Start Final Capto MMC ImpRes 9 0.3 4.5 26 1 Capto SP ImpRes 8 0.5 4. In this study, we demonstrated that when Capto MMC ImpRes chromatography is conducted under weak partitioning mode, high throughput, good yield, and effective byproduct removal are simultaneously achieved.īispecific antibody (bsAb) Capto MMC ImpRes Light chain (LC)-missing byproduct Mixed-mode chromatography Weak partitioning mode WuXiBody.Ĭopyright © 2021 Elsevier Inc. Capto MMC ImpRes (Fig 1) is a BioProcess chromatography medium (resin) for high-resolution polishing of monoclonal. However, the dynamic binding capacity (DBC) of Capto MMC ImpRes is relatively low under the selected condition, making the process less desirable for large-scale manufacturing. We previously showed that Capto MMC ImpRes mixed-mode chromatography under bind-elute mode can effectively remove this LC-missing species. In this chapter, we describe a procedure for antibody aggregate removal using the mixed-mode resin Capto MMC ImpRes, whose ligand mediates ionic interactions, hydrophobic interactions, and hydrogen bonding 15, 16. The high chemical stability of Capto resins lets you perform cleaning-in-place procedures with ease. While for bsAbs based on other platforms removal of such byproduct can pose considerable challenge to the downstream team, in this case WuXiBody's unique design makes separation relatively straightforward. EXPLORE PRODUCTS Gain 95 productivity in chromatography operations Capto chromatography resins give you increased productivity compared with Sepharose resins, through high binding capacity and high flow rates. While the multimodal anion exchangers exhibited significant recovery issues, the Capto MMC and MMC ImpRes resin systems were observed to have good recoveries and some selectivity for these challenging separations under linear gradient conditions. For purifying a WuXiBody-based symmetric bsAb, we identified a LC-missing species as a major byproduct. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. It has a different selectivity, compared with traditional ion exchangers. 1 Packing factor is defined as: consolidated bed height/packed bed height. Capto MMC is a multimodal weak cation exchanger that is salt-tolerant. ![]() Its key feature is the replacement of one parental antibody's CH1/CL region with the T cell receptor (TCR) constant domain, a design that promotes cognate heavy chain (HC)-light chain (LC) pairing. AxiChrom columns are low-pressure, axial compression. WuXiBody is a bispecific antibody (bsAb) platform developed by WuXi Biologics. ![]()
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